Action of lipase on milk

Shopping: 500cm3 full cream milk

Apparatus

Water baths at 36C
1 and 3 cm3 pipettes

Materials


5% Bile Salts (25cm3 per group)

100cm3 Phenolphthalein - diluted to half strength with ethanol (5cm3 per group)

2% Lipase (5cm3 per group)

"Alkaline milk" - 50cm3 full cream milk and 10cm3 0.2M Na2CO3
(1M Na2CO3 = 286.15 gl-1 so 0.2M = 57.2 gl-1)